SH1911
Sigma
MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock
Apoptosis Pathway
Description
| General description | The clones are sequenced-verified shRNA lentiviral plasmids (pLKO.1-puro) provided as 50 μl bacterial glycerol stocks (Terrific Broth, carbenicillin at 100 μg/ml and 15% glycerol). The set comes in 96-well plates that are barcoded for simple identification. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set. |
| Other Notes | The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated. |
| Number of Genes: 440, Number of Clones: 2882 | |
| For a detailed listing of other available gene family sets, visit the gene family set website. | |
| Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene. | |
| Legal Information | Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense . |
| MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| storage temp. | −70°C |
References
| reference | Demedts, I. K., et al., Role of apoptosis in the pathogenesis of COPD and pulmonary emphysema Respir. Med. 7, 53, (2006) |
Fink, S. K., et al., Apoptosis, Pyroptosis, and Necrosis: Mechanistic Description of Dead and Dying Eukaryotic Cells Infect. Immun. 4th ed., 73, 1907-1916, (2005) Abstract | |
| Miller, M. A., et al., Caspase 8L, a novel inhibitory isoform of caspase 8, is associated with undifferentiated neuroblastoma Apoptosis 11, 15-24 , (2006) Abstract | |
| Krysko, D. V., et al., Apoptosis and necrosis: detection, discrimination and phagocytosis Methods 44, 205-221, (2008) Abstract | |
| Logue, S. E., and Martin, S. J., Caspase activation cascades in apoptosis Biochem. Soc. Trans. 36, 1-9, (2008) Abstract |
